Antibody data

Product number
11-0497-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Integrin beta 5 Monoclonal Antibody (KN52), FITC, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The KN52 antibody reacts with human integrin beta 5, an approximately 100,000 Mr (reduced) and 95,000 Mr (nonreduced) a member of the integrin family. Only a single alpha chain, the alpha v subunit, associates with the integrin beta 5 to form the vitronectin receptor complex. Integrin alpha v/beta 5 complex also binds to the basic domain of Tat (he sequence RKKRRQRRR). The integrin beta 5 is found on carcinoma cell lines, hepatoma and fibroblast cell lines, and is absent from lymphoblastoid cells and platelets. The KN52 monoclonal antibody has been shown to inhibit migration. Applications Reported: This KN52 antibody has been reported for use in flow cytometric analysis. Applications Tested: This KN52 antibody has been pre-titrated and tested by flow cytometric analysis of Chinese Hamster Ovary (CHO), A549 and normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse, Hamster
Host
Mouse
Conjugate
Green dye
Isotype
IgG
Antibody clone number
KN52
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
ITGB5 protein structure - 11-0497-41 shown in red.