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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- FAB990A - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal SIGIRR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human SIGIRR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) IL-1 RI, rhIL-1 R3, rhIL-1 R8, rhIL-1 RII, rhIL-1 R6, rhIL-1 R4, rhIL-18, rhIL-1 R7, rhIL-9 R9, or recombinant mouse SIGIRR.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of SIGIRR in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human SIGIRR APC-conjugated Monoclonal Antibody (Catalog # FAB990A) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
