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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP2-32274 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal FAT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FAT1 Antibody [NBP2-32274] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-FAT1 antibody NBP2-32274 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FAT1 Antibody [NBP2-32274] - Whole cell lysate (50 ug) from Hep-G2, RKO, HeLa, Jurkat, HEK293T, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAT1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: FAT1 Antibody [NBP2-32274] - Detection of human FAT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FAT1 antibody NBP2-32274 (lot NBP2-32274-2) used for IP at 6 ug per reaction. FAT1 was also immunoprecipitated by a previous lot of this antibody (lot NBP2-32274-1) and rabbit anti-FAT1 antibody NBP2-32275. For blotting immunoprecipitated FAT1, NBP2-32274 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
