- PA1-957 - Invitrogen Antibodies TNFRSF10B antibody

Submitted references Oxaliplatin resistance in colorectal cancer enhances TRAIL sensitivity via death receptor 4 upregulation and lipid raft localization.

Greenlee JD, Lopez-Cavestany M, Ortiz-Otero N, Liu K, Subramanian T, Cagir B, King MR
eLife 2021 Aug 3;10

HIF-1α Mediates TRAIL-Induced Neuronal Apoptosis via Regulating DcR1 Expression Following Traumatic Brain Injury.

Fang Y, Lu J, Wang X, Wu H, Mei S, Zheng J, Xu S, Lenahan C, Chen S, Zhang J, Hong Y
Frontiers in cellular neuroscience 2020;14:192

BCL2L10 protein regulates apoptosis/proliferation through differential pathways in gastric cancer cells.

Xu JD, Cao XX, Long ZW, Liu XP, Furuya T, Xu JW, Liu XL, De Xu Z, Sasaki K, Li QQ
The Journal of pathology 2011 Feb;223(3):400-9

Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors.

Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A
Science (New York, N.Y.) 1997 Aug 8;277(5327):818-21

Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors.

Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A
Science (New York, N.Y.) 1997 Aug 8;277(5327):818-21

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TRAIL-R2/DR5 was done on 70% confluent log phase HeLa cell. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TRAIL-R2/Rabbit Polyclonal Antibody (Product # PA1-957) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TRAIL-R2 was performed using HCT 116 cells and HCT 116 treated with Thapsigargin (1 µM, 36 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TRAIL-R2 Rabbit Polyclonal Antibody (Product # PA1-957) at 2 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in HCT 116 treated cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HCT116 treated cells, which shows higher expression for TRAIL-R2 protein showing localization in nucleus, cytoplasm and membrane. Panel e represents the merged image of untreated HCT 116 cells, that shows lower or no expression for TRAIL-R2 protein. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TRAIL-R2/DR5 was done on 70% confluent log phase HeLa cell. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TRAIL-R2/Rabbit Polyclonal Antibody (Product # PA1-957) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TRAIL-R2 was performed using HCT 116 cells and HCT 116 treated with Thapsigargin (1 µM, 36 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TRAIL-R2 Rabbit Polyclonal Antibody (Product # PA1-957) at 2 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in HCT 116 treated cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HCT116 treated cells, which shows higher expression for TRAIL-R2 protein showing localization in nucleus, cytoplasm and membrane. Panel e represents the merged image of untreated HCT 116 cells, that shows lower or no expression for TRAIL-R2 protein. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of TRAIL-R2 / DR5 was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with TRAIL-R2 / DR5 Rabbit Polyclonal Antibody (PA1957, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Protein expression level and cellular localization of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and death receptor 5 (DR5). (A) Representative picture of the traumatic brain injury (TBI) model. (B) Representative Western blot bands of each protein. (C) Densitometric quantification of TRAIL. (D) Densitometric quantification of DR5. (E) Densitometric quantification of decoy receptor 1 (DcR1). (F) Densitometric quantification of DcR2. (G) Representative microphotographs of immunofluorescence staining showing localization of TRAIL and DR5 (red), Iba-1 and NeuN (green) in injured cerebral cortex after TBI. Scale bar = 50 mum. (H) Quantification of TRAIL-positive cells in sham group and TBI group. (I) Quantification of DR5-positive cells in sham group and TBI group. Scale bar = 50 mum; N = 6 per group. Data are represented as mean +- SD. * p < 0.05 vs. sham; ns, no significance vs. sham. One-way ANOVA, Tukey's post hoc test .

PA1-957

Antibody data