PA5-19895

antibody from Invitrogen Antibodies

Targeting: TNFRSF10B CD262, DR5, KILLER, TRAIL-R2, TRAILR2, TRICK2A, TRICKB

Antibody data

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Validation data

Product number: PA5-19895 - Provider product page
Provider: Invitrogen Antibodies
Product name: TRAIL-R2 (DR5) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: A suggested positive control is Hela cell lysate. PA5-19895 can be used with blocking peptide PEP-0021. The PA5-19895 immunogen is located within the last 50 amino acids of DR5. Predicted molecular ~ 48kD and 45kD. In Western blot applications, this antibody has been observed to detect a band at: 48kD and 45kD
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4° C

TNFRSF10B protein structure - PA5-19895 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HeLa (H) and K562 (K) cell lysates using a CD262/TRAIL Receptor 2 DR5 polyclonal antibody (Product # PA5-19895) at 2 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) (0.5 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Human HepG2 Cells. Loading: 15 µg of lysates per lane. Antibodies: TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 µg/mL Lane 2: 2 µg/mL Lane 3: 4 µg/mL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) (2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Mouse Heart. Loading: 15 µg of lysatesper lane. Antibodies: TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Rat Skeletal Muscle. Loading: 15 µg of lysate per lane. Antibodies: TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry staining of HeLa cells using a CD262/TRAIL Receptor 2 DR5 polyclonal antibody (Product # PA5-19895) at a 5 µg/mL dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling DR5 with TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain tissue labeling DR5 with TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling DR5 with TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling DR5 with TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain tissue labeling DR5 with TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using TRAIL-R2 (DR5) Polyclonal Antibody (Product # PA5-19895) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.