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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00008780-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008780-M01, RRID:AB_425783
- Product name
- RIOK3 monoclonal antibody (M01), clone 4A12-1D2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant RIOK3.
- Antigen sequence
MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSL
ADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGE
NIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNG
DSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDD
PYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKN
TARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHA
YSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMV
NSGMLETITGCISTGKESVVFHAYGGSMEDEKEDS
KVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDR
FSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVV
LLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEA
YYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVW
LIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEA
LSERELFNAVSGLNITADNEADFLAEIEALEKMNE
DHVQKNGRKAASFLKDDGDPPLLYDE- Isotype
- IgG
- Antibody clone number
- 4A12-1D2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Hypoxic regulation of RIOK3 is a major mechanism for cancer cell invasion and metastasis.
Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer.
Singleton DC, Rouhi P, Zois CE, Haider S, Li JL, Kessler BM, Cao Y, Harris AL
Oncogene 2015 Sep 3;34(36):4713-22
Oncogene 2015 Sep 3;34(36):4713-22
Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer.
Kimmelman AC, Hezel AF, Aguirre AJ, Zheng H, Paik JH, Ying H, Chu GC, Zhang JX, Sahin E, Yeo G, Ponugoti A, Nabioullin R, Deroo S, Yang S, Wang X, McGrath JP, Protopopova M, Ivanova E, Zhang J, Feng B, Tsao MS, Redston M, Protopopov A, Xiao Y, Futreal PA, Hahn WC, Klimstra DS, Chin L, DePinho RA
Proceedings of the National Academy of Sciences of the United States of America 2008 Dec 9;105(49):19372-7
Proceedings of the National Academy of Sciences of the United States of America 2008 Dec 9;105(49):19372-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M01), clone 4A12-1D2.Lane 1: RIOK3 transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RIOK3 monoclonal antibody (M01), clone 4A12-1D2. Western Blot analysis of RIOK3 expression in MCF-7.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RIOK3 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of RIOK3 transfected lysate using anti-RIOK3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RIOK3 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
