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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-29733 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FECH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2. Predicted reactivity: Mouse (92%), Rat (93%), Xenopus laevis (87%), Pig (89%), Chicken (85%), Chimpanzee (100%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.74 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FerrocHeLatase using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FerrocHeLatase polyclonal antibody (Product # PA5-29733) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FECH was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FECH Polyclonal Antibody (Product # PA5-29733) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
