PAB28656

antibody from Abnova Corporation

Targeting: SH2D4A FLJ20967, PPP1R38, SH2A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28656

Provider

Abnova Corporation

Product name

SH2D4A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SH2D4A.

Antigen sequence

KEEQLPLRAGYQKTSDTIAPWFHGILTLKKANELL
LSTGMPGSFLIRVSERIKGYALSYLSEDGCKHFLI
DASADAYSFLGVDQLQHATLADLVEYHKEEPITSL
GKELLLYPCGQQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Mouse liver tissue lysate, Lane 2: Rat liver tissue lysate with SH2D4A polyclonal antibody (Cat # PAB28656) at 1:100-1:500 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: A-431, Lane 4: Human liver with SH2D4A polyclonal antibody (Cat # PAB28656) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with SH2D4A polyclonal antibody (Cat # PAB28656) at 1-4 ug/mL shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human liver with SH2D4A polyclonal antibody (Cat # PAB28656) shows strong cytoplasmic positivity in hepatocytes at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)