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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- GTX85830 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX85830, RRID:AB_10733561
- Product name
- RREB1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of Protein A Purified anti-RREB1 antibody shows detection of a predominant band believed to be RREB1 in various cell lysates (1 - HEK293, 2 - RFP-RREB transfected HEK293, 3 - M460 and 4 - T1165). All lysates were loaded at 20 ug per lane and separated by SDS-PAGE. After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1000. The membrane was washed and reacted with IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX. Size estimation was made by comparison to prestained MW markers as indicated.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-RREB1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody GTX85830 concentration 10 μg/ml.
