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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- RQ6582 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NIRF Antibody / HRF2
- Antibody type
- Monoclonal
- Description
- This highly specific NIRF antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 6B5
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NIRF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HepG2, 2) HT1080 and 3) Jurkat cell lysate with NIRF antibody. Expected molecular weight ~90 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with NIRF antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with NIRF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NIRF antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of rat RH35 cells with NIRF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NIRF antibody.
