MA5-49293

antibody from Invitrogen Antibodies

Targeting: UHRF2 MGC33463, NIRF, RNF107, TDRD23, URF2

Western blot

Antibody data

Submit

Validation data

Product number: MA5-49293 - Provider product page
Provider: Invitrogen Antibodies
Product name: UHRF2 Monoclonal Antibody (6B5)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL. Immunogen sequence is identical to the related mouse and rat sequences. Positive Control - WB: human HepG2 whole cell, human HT1080 whole cell, human Jurkat whole cell. ICC/IF: Hela cell. Flow: Hela cell, RH35 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Reactivity: Human, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 6B5
Vial size: 100 µg
Concentration: 500 µg/mL
Storage: -20°C

UHRF2 protein structure - MA5-49293 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of UHRF2 in Hela cells using UHRF2 Monoclonal Antibody (6B5) (Product # MA5-49293), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-mouse IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of UHRF2 in RH35 cells (Blue line). The cells were blocked with 10% normal goat serum, and then incubated with UHRF2 monoclonal antibody (Product # MA5-49293) (1 µg/1x10^6 cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10^6 cells) used under the same conditions. Unlabeled sample (Red line) was also used as a control. DyLight®488 conjugated goat anti-mouse IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of UHRF2 in RH35 cells using UHRF2 Monoclonal Antibody (6B5) (Product # MA5-49293), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-mouse IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.