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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [3]
- Immunohistochemistry [1]
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- Product number
- R20190 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Human IgG4 Antibody / Hinge region
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Human IgG4 antibody is suitable for use in Immunocytochemistry/Immunohistochemistry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM120
- Vial size
- 100 ug
- Concentration
- 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant Human IgG4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of human, mouse, rat, and goat IgG shows the recombinant Human IgG4 antibody reacts to hIgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of hIgG, or mouse/rat/goat IgG.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA with human plasma using recombinant Human IgG4 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA with human IgG4 using recombinant Human IgG4 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lymphoid tissue with recombinant Human IgG4 antibody.
