R20190BTN

antibody from NSJ Bioreagents

Targeting: IGHG4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R20190BTN

Provider

NSJ Bioreagents

Product name

Recombinant Human IgG4 Antibody (Hinge region) Biotin Conjugate

Antibody type

Monoclonal

Description

This highly specific Recombinant Human IgG4 antibody is suitable for use in Immunocytochemistry/Immunohistochemistry/ELISA applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RM120

Vial size

50 ug

Concentration

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant Human IgG4 antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot of human, mouse, rat, and goat IgG shows the recombinant Human IgG4 antibody reacts to hIgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of hIgG, or mouse/rat/goat IgG.

ELISA

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA with human IgG4 using recombinant Human IgG4 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA with human plasma using recombinant Human IgG4 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lymphoid tissue with recombinant Human IgG4 antibody.