R20191BTN

antibody from NSJ Bioreagents

Targeting: IGHG4

ELISA (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

R20191BTN

Provider

NSJ Bioreagents

Product name

Recombinant Human IgG4 Fc Antibody

Antibody type

Recombinant

Description

Purified

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Antibody clone number

RM217

Vial size

50 µg

Concentration

1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant Human IgG4 Fc antibody at -20°C.

ELISA

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA with human IgG4 using recombinant Human IgG4 Fc antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA with human plasma using recombinant Human IgG4 Fc antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 Fc antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Recombinant Human IgG4 Fc antibody specifically reacts to hIgG4. No cross reactivity with human IgG1, IgG2, IgG3.