ALX-804-344A-C100

antibody from Enzo Life Sciences

Targeting: TNFRSF10C CD263, DcR1, LIT, TRAILR3, TRID

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ALX-804-344A-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-344A-C100, RRID:AB_2052728

Product name

TRAIL-R3 (human) monoclonal antibody (HS301)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

HS301

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Resistance to FasL and tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in Sezary syndrome T-cells associated with impaired death receptor and FLICE-inhibitory protein expression.

Contassot E, Kerl K, Roques S, Shane R, Gaide O, Dupuis M, Rook AH, French LE
Blood 2008 May 1;111(9):4780-7

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TRAIL/bortezomib cotreatment is potentially hepatotoxic but induces cancer-specific apoptosis within a therapeutic window.

Koschny R, Ganten TM, Sykora J, Haas TL, Sprick MR, Kolb A, Stremmel W, Walczak H
Hepatology (Baltimore, Md.) 2007 Mar;45(3):649-58

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Enhanced caspase-8 recruitment to and activation at the DISC is critical for sensitisation of human hepatocellular carcinoma cells to TRAIL-induced apoptosis by chemotherapeutic drugs.

Ganten TM, Haas TL, Sykora J, Stahl H, Sprick MR, Fas SC, Krueger A, Weigand MA, Grosse-Wilde A, Stremmel W, Krammer PH, Walczak H
Cell death and differentiation 2004 Jul;11 Suppl 1:S86-96

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TRAIL regulates normal erythroid maturation through an ERK-dependent pathway.

Secchiero P, Melloni E, Heikinheimo M, Mannisto S, Di Pietro R, Iacone A, Zauli G
Blood 2004 Jan 15;103(2):517-22

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cFLIPL inhibits tumor necrosis factor-related apoptosis-inducing ligand-mediated NF-kappaB activation at the death-inducing signaling complex in human keratinocytes.

Wachter T, Sprick M, Hausmann D, Kerstan A, McPherson K, Stassi G, Bröcker EB, Walczak H, Leverkus M
The Journal of biological chemistry 2004 Dec 17;279(51):52824-34

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TNF-related apoptosis-inducing ligand mediates tumoricidal activity of human monocytes stimulated by Newcastle disease virus.

Washburn B, Weigand MA, Grosse-Wilde A, Janke M, Stahl H, Rieser E, Sprick MR, Schirrmacher V, Walczak H
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 15;170(4):1814-21

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Receptor specificity ofthe monoclonal antibodies HS301 for TRAIL-R3 (Prod. No. ALX-804-344). Method: CV1/EBNA cells were transfected either with the empty expression vector pCDNA3 (vector control) or pCDNA3 encodingTRAIL-R1 to TRAIL-R4.Two days after transfection 1 x 10E6 cells were stained with mAb HS301 against TRAIL-R3 (Prod. No. ALX- 804-344). . All primary antibodies were used at 10 µg/ml, followed by biotinylated goat anti-mouse IgG1 and streptavidin-PE. Transfection efficiency was about 50% in all individual transfections. Note: HS301 also detects endogenous cell surface expressed TRAIL-R3 in FACS (unpublished data). In most cell types endogenous TRAIL-R3 appears to be mainly expressed intracellular, why cell permeabilization may be required to obtain a signal by FACS.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

TRAIL-R3 endogenous expression analysis by various MAbs.