NBP2-25219

antibody from Novus Biologicals

Targeting: PODXL Gp200, PC, PCLP

Immunocytochemistry

Immunoprecipitation

Antibody data

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Validation data

Product number: NBP2-25219 - Provider product page
Provider: Novus Biologicals
Product name: Mouse Monoclonal Podocalyxin Like Antibody
Antibody type: Monoclonal
Description: Protein A or G purified. Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
Reactivity: Human, Mouse, Rat, Rabbit
Host: Mouse
Isotype: IgG
Vial size: 0.1 mg
Concentration: 0.2 mg/ml
Storage: Store at 4C.

PODXL protein structure - NBP2-25219 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Analysis using PE conjugate of NBP2-25219. A cell surface stain was performed on HeLa cells with Podocalyxin antibody (3D3) NBP2-33108 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Analysis of Allophycocyanin conjugate of NBP2-25219. An intracellular stain was performed on HeLa cells with Podocalyxin (3D3) antibody NBP2-33108APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permea

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Surface staining of NCCIT cells using Podocalyxin antibody at 1 ug/10^6 cells. A FITC-conjugated anti mouse Ig secondary antibody was used along with this antibody .

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Analysis of Allophycocyanin conjugate of NBP2-25219. An intracellular stain was performed on SH-SY5Y cells with Podocalyxin (3D3) antibody NBP2-33108APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then per

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): Podocalyxin Like Antibody (3D3) [NBP2-25219] - Flow Cytometry: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - An intracellular stain was performed on HeLa cells with Podocalyxin Like Antibody (3D3) NBP2-33108PE and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the Azide and BSA Free form of this antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): Podocalyxin Like Antibody (3D3) [NBP2-25219] - An intracellular stain was performed on HeLa cells with Podocalyxin Like Antibody (3D3) NBP2-33108APC and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.