Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AM2150a - Provider product page
- Provider
- Abgent
- Product name
- PODXL Antibody (C-term) (Ascites)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- PODXL Antibody (C-term)(Ascites)(Cat. #AM2150a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PODXL antibody detected the PODXL protein (arrow).
- Primary Ab dilution
- 1:500~16000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent confocal image of SY5Y cells stained with PODXL (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2150a PODXL (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). "
- Primary Ab dilution
- 1:100