12-8873-41

antibody from Invitrogen Antibodies
Targeting: PODXL Gp200, PC, PCLP
Provider product page for 12-8873-41

Antibody data

Product number
12-8873-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-Podocalyxin Monoclonal Antibody (B34D1.3), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody B34D1.3 recognizes human podocalyxin, a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). Podocalxyin is a heavily glycosylated cell adhesion protein with homology to CD34 and endoglycan. It was first identified on kidney podocytes, and then found to be upregulated in a variety of human cancers such as breast and prostate cancer. Like other stem cell-specific markers, the epitope recognized by the B34D1.3 antibody is lost upon cell differentiation. The B34D1.3 antibody recognizes the ectodomain of podocalyxin, which is a different epitope from the Tra-1-60 and Tra-1-81 monoclonal antibodies. While Tra1-60 has been found to react with a sialidase-sensitive epitope, the epitope recognized by Tra1-81 has not been fully defined. Applications Reported: This B34D1.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This B34D1.3 antibody has been pre-titrated and tested by flow cytometric analysis of the NTERA cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
B34D1.3
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
PODXL protein structure - 12-8873-41 shown in red.