Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-8873-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Podocalyxin Monoclonal Antibody (B34D1.3), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody B34D1.3 recognizes human podocalyxin, a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). Podocalxyin is a heavily glycosylated cell adhesion protein with homology to CD34 and endoglycan. It was first identified on kidney podocytes, and then found to be upregulated in a variety of human cancers such as breast and prostate cancer. Like other stem cell-specific markers, the epitope recognized by the B34D1.3 antibody is lost upon cell differentiation. The B34D1.3 antibody recognizes the ectodomain of podocalyxin, which is a different epitope from the Tra-1-60 and Tra-1-81 monoclonal antibodies. While Tra1-60 has been found to react with a sialidase-sensitive epitope, the epitope recognized by Tra1-81 has not been fully defined. Applications Reported: This B34D1.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This B34D1.3 antibody has been pre-titrated and tested by flow cytometric analysis of the NTERA cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- B34D1.3
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Modeling of FAN1-Deficient Kidney Disease Using a Human Induced Pluripotent Stem Cell-Derived Kidney Organoid System.
The role of podocalyxin in health and disease.
The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma.
Production and characterization of murine monoclonal antibodies against human podocalyxin.
Lim SW, Na D, Lee H, Fang X, Cui S, Shin YJ, Lee KI, Lee JY, Yang CW, Chung BH
Cells 2023 Sep 20;12(18)
Cells 2023 Sep 20;12(18)
The role of podocalyxin in health and disease.
Nielsen JS, McNagny KM
Journal of the American Society of Nephrology : JASN 2009 Aug;20(8):1669-76
Journal of the American Society of Nephrology : JASN 2009 Aug;20(8):1669-76
The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma.
Schopperle WM, DeWolf WC
Stem cells (Dayton, Ohio) 2007 Mar;25(3):723-30
Stem cells (Dayton, Ohio) 2007 Mar;25(3):723-30
Production and characterization of murine monoclonal antibodies against human podocalyxin.
RodrÃguez RB, Butta N, Larrucea S, Alonso S, Parrilla R
Tissue antigens 2006 Nov;68(5):407-17
Tissue antigens 2006 Nov;68(5):407-17
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the NTERA cell line with Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (blue histogram) or Anti-Human Podocalyxin PE (purple histogram). Total viable cells were used for analysis.
- Conjugate
- Yellow dye