M03359

antibody from Boster Biological Technology

Targeting: PODXL Gp200, PC, PCLP

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M03359

Provider

Boster Biological Technology

Product name

Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody

Antibody type

Monoclonal

Description

Mouse Monoclonal Antibody [Clone 3D3] antibody for Podocalyxin/PODXL detection. Host: Mouse.Size: 100ug/vial. Tested applications: Flow Cytometry. Reactive species: Human. Podocalyxin/PODXL information: Molecular Weight: 58635 MW; Subcellular Localization: Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus . Membrane raft . Membrane ; Single-pass type I membrane protein . In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans- Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity); Tissue Specificity: Glomerular epithelium cell (podocyte).

Reactivity

Human, Rat, Rabbit

Host

Mouse

Antibody clone number

3D3

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.