330606

antibody from BioLegend
Targeting: PODXL Gp200, PC, PCLP
Provider product page for 330606

Antibody data

Product number
330606 - Provider product page
Provider
BioLegend
Proper citation
BioLegend Cat#330606, RRID:AB_1227812
Product name
Alexa Fluor® 647 anti-human TRA-1-60-R [TRA-1-60-R]
Antibody type
Monoclonal
Antigen
Human embryonal carinoma cell line 2102Ep cl.2A6
Description
TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R).  The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity.  Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.
Reactivity
Human
Host
Mouse
Conjugate
Red dye
Isotype
IgM
Vial size
100 tests
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PODXL protein structure - 330606 shown in red.