Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 330705 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#330705, RRID:AB_1089245
- Product name
- Alexa Fluor® 647 anti-human TRA-1-81 [TRA-1-81]
- Antibody type
- Monoclonal
- Antigen
- Human embryonic carcinoma cell line 2102Ep cl.2A6
- Description
- The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgM
- Vial size
- 25 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- NCCIT (human teratocarcinoma cell line) stained with TRA-1-81 Alexa Fluor® 647
- Conjugate
- Red dye