Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-83348 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83348, RRID:AB_11003823
- Product name
- Rabbit Polyclonal Podocalyxin Like Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Podocalyxin Like antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Value of immunohistochemical expression of podocalyxin in active lupus nephritis.
Prognostic and predictive significance of podocalyxin-like protein expression in pancreatic and periampullary adenocarcinoma.
Podocalyxin Is a Marker of Poor Prognosis in Pancreatic Ductal Adenocarcinoma.
Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays.
Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer.
Sialofucosylated podocalyxin is a functional E- and L-selectin ligand expressed by metastatic pancreatic cancer cells.
Methylation of an intronic region regulates miR-199a in testicular tumor malignancy.
Overexpression of podocalyxin-like protein is an independent factor of poor prognosis in colorectal cancer.
Behairy MA, Shakweer MM, El Said TW, ElGharbawy NH
Nefrologia : publicacion oficial de la Sociedad Espanola Nefrologia 2018 Jan - Feb;38(1):64-72
Nefrologia : publicacion oficial de la Sociedad Espanola Nefrologia 2018 Jan - Feb;38(1):64-72
Prognostic and predictive significance of podocalyxin-like protein expression in pancreatic and periampullary adenocarcinoma.
Heby M, Elebro J, Nodin B, Jirström K, Eberhard J
BMC clinical pathology 2015;15:10
BMC clinical pathology 2015;15:10
Podocalyxin Is a Marker of Poor Prognosis in Pancreatic Ductal Adenocarcinoma.
Saukkonen K, Hagström J, Mustonen H, Juuti A, Nordling S, Fermér C, Nilsson O, Seppänen H, Haglund C
PloS one 2015;10(6):e0129012
PloS one 2015;10(6):e0129012
Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays.
Forsström B, Axnäs BB, Stengele KP, Bühler J, Albert TJ, Richmond TA, Hu FJ, Nilsson P, Hudson EP, Rockberg J, Uhlen M
Molecular & cellular proteomics : MCP 2014 Jun;13(6):1585-97
Molecular & cellular proteomics : MCP 2014 Jun;13(6):1585-97
Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer.
Boman K, Larsson AH, Segersten U, Kuteeva E, Johannesson H, Nodin B, Eberhard J, Uhlén M, Malmström PU, Jirström K
British journal of cancer 2013 Jun 11;108(11):2321-8
British journal of cancer 2013 Jun 11;108(11):2321-8
Sialofucosylated podocalyxin is a functional E- and L-selectin ligand expressed by metastatic pancreatic cancer cells.
Dallas MR, Chen SH, Streppel MM, Sharma S, Maitra A, Konstantopoulos K
American journal of physiology. Cell physiology 2012 Sep 15;303(6):C616-24
American journal of physiology. Cell physiology 2012 Sep 15;303(6):C616-24
Methylation of an intronic region regulates miR-199a in testicular tumor malignancy.
Cheung HH, Davis AJ, Lee TL, Pang AL, Nagrani S, Rennert OM, Chan WY
Oncogene 2011 Aug 4;30(31):3404-15
Oncogene 2011 Aug 4;30(31):3404-15
Overexpression of podocalyxin-like protein is an independent factor of poor prognosis in colorectal cancer.
Larsson A, Johansson ME, Wangefjord S, Gaber A, Nodin B, Kucharzewska P, Welinder C, Belting M, Eberhard J, Johnsson A, Uhlén M, Jirström K
British journal of cancer 2011 Aug 23;105(5):666-72
British journal of cancer 2011 Aug 23;105(5):666-72
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Podocalyxin Like Antibody [NBP1-83348] - Analysis in human kidney tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Podocalyxin Like Antibody [NBP1-83348] - Analysis in control (vector only transfected HEK293T lysate) and PODXL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human kidney shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Analysis in human kidney and liver tissues. Corresponding Podocalyxin Like RNA-seq data are presented for the same tissues.