MA1-024

antibody from Invitrogen Antibodies

Targeting: PODXL Gp200, PC, PCLP

Western blot

Antibody data

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Validation data

PODXL protein structure - MA1-024 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows staining on the cell surface of human H9 human embryonic stem cells, indicating pluripotency. TRA-1-81 staining (green) and an overlay image of TRA-1-81 with DAPI (blue) is shown. H9 human embryonic stem cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-81 (Product # MA1-024) at a dilution of 1:20 overnight at 4 °C, washed with PBS and incubated with a FITC-conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows expression in human teratocarcinoma NCCIT cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing TRA-1-81 (Product # MA1-024), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with goat-anti-mouse IgM secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows staining on the cell surface of human HEL 11.4 iPS cells, indicating pluripotency. TRA-1-81 staining (green) and an overlay image of TRA-1-81 with DAPI (blue) is shown. HEL 11.4 iPS cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-81 (Product # MA1-024) at a dilution of 1:20 overnight at 4 °C, washed with PBS and incubated with a FITC-conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of TRA-1-81 was performed on NCCIT cells. NCCIT cells were harvested, fixed and washed with PBS. Cells were incubated with 0.4 µg of TRA-1-60 monoclonal antibody (Product # MA1-024), followed by incubation with FITC-conjugated goat-anti-mouse IgM secondary antibody (Product # 31992), indicated by the shifted peak (blue line). To control for non-specific binding, cells were also incubated with secondary antibody alone (black line). Incubations were performed for 30 mins in the dark and 10,000 cells were analyzed for each sample.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows positive staining of H9 human embryonic stem cells (blue line). H9 human embryonic stem cells were harvested, fixed and washed with PBS. Cells were incubated with anti-TRA-1-81 monoclonal antibody (Product # MA1-024) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with FITC-conjugated secondary antibody. 100,000 cells were stained for each sample.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows positive staining of HEL 11.4 iPS cells (blue line). HEL 11.4 iPS cells were harvested, fixed and washed with PBS. Cells were incubated with anti-TRA-1-81 monoclonal antibody (Product # MA1-024) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with FITC-conjugated secondary antibody. 100,000 cells were stained for each sample.