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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-28116 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PODXL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: human ESC. Predicted reactivity: Dog (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references R-Ras-Akt axis induces endothelial lumenogenesis and regulates the patency of regenerating vasculature.
Li F, Sawada J, Komatsu M
Nature communications 2017 Nov 23;8(1):1720
Nature communications 2017 Nov 23;8(1):1720
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PODXL using 20 µg of human ESC lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PODXL polyclonal antibody (Product # PA5-28116) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PODXL in methanol-fixed mouse ESC cells using a PODXL polyclonal antibody (Product # PA5-28116) at a 1:100 dilution.
