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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- NB100-730 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-730, RRID:AB_10001809
- Product name
- PODXL Antibody (TRA-1-60(R)) - Embryonic Stem Cell Marker
- Antibody type
- Monoclonal
- Antigen
- Human embryonal carcinoma cell line 2102Ep.
- Host
- Mouse
- Isotype
- IgM
- Concentration
- 4.1 mg/ml
Submitted references Liver biopsy derived induced pluripotent stem cells provide unlimited supply for the generation of hepatocyte-like cells.
The sTRA Plasma Biomarker: Blinded Validation of Improved Accuracy Over CA19-9 in Pancreatic Cancer Diagnosis.
Generation of human induced pluripotent stem cell lines (hiPSC) from one bipolar disorder patient carrier of a DGKH risk haplotype and one non-risk-variant-carrier bipolar disorder patient.
Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using integration free Sendai virus technology.
Corrigendum to "Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector" [Stem Cell Res. 16/2 (2016) 314-317].
Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector.
Glycans related to the CA19-9 antigen are elevated in distinct subsets of pancreatic cancers and improve diagnostic accuracy over CA19-9.
Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells.
The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.
Calabrese D, Roma G, Bergling S, Carbone W, Mele V, Nuciforo S, Fofana I, Campana B, Szkolnicka D, Hay DC, Tchorz J, Bouwmeester T, Wieland S, Heim MH
PloS one 2019;14(8):e0221762
PloS one 2019;14(8):e0221762
The sTRA Plasma Biomarker: Blinded Validation of Improved Accuracy Over CA19-9 in Pancreatic Cancer Diagnosis.
Staal B, Liu Y, Barnett D, Hsueh P, He Z, Gao C, Partyka K, Hurd MW, Singhi AD, Drake RR, Huang Y, Maitra A, Brand RE, Haab BB
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 May 1;25(9):2745-2754
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 May 1;25(9):2745-2754
Generation of human induced pluripotent stem cell lines (hiPSC) from one bipolar disorder patient carrier of a DGKH risk haplotype and one non-risk-variant-carrier bipolar disorder patient.
Palladino VS, Subrata NOC, Geburtig-Chiocchetti A, McNeill R, Hoffmann P, Reif A, Kittel-Schneider S
Stem cell research 2018 Oct;32:104-109
Stem cell research 2018 Oct;32:104-109
Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using integration free Sendai virus technology.
Rossbach B, Hildebrand L, El-Ahmad L, Stachelscheid H, Reinke P, Kurtz A
Stem cell research 2017 May;21:167-170
Stem cell research 2017 May;21:167-170
Corrigendum to "Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector" [Stem Cell Res. 16/2 (2016) 314-317].
Rossbach B, Hildebrand L, El-Ahmad L, Stachelscheid H, Reinke P, Kurtz A
Stem cell research 2016 Sep;17(2):461
Stem cell research 2016 Sep;17(2):461
Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector.
Rossbach B, Hildebrand L, El-Ahmad L, Stachelscheid H, Reinke P, Kurtz A
Stem cell research 2016 Mar;16(2):314-7
Stem cell research 2016 Mar;16(2):314-7
Glycans related to the CA19-9 antigen are elevated in distinct subsets of pancreatic cancers and improve diagnostic accuracy over CA19-9.
Tang H, Partyka K, Hsueh P, Sinha JY, Kletter D, Zeh H, Huang Y, Brand RE, Haab BB
Cellular and molecular gastroenterology and hepatology 2016 Feb 1;2(2):201-221.e15
Cellular and molecular gastroenterology and hepatology 2016 Feb 1;2(2):201-221.e15
Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells.
Matin MM, Walsh JR, Gokhale PJ, Draper JS, Bahrami AR, Morton I, Moore HD, Andrews PW
Stem cells (Dayton, Ohio) 2004;22(5):659-68
Stem cells (Dayton, Ohio) 2004;22(5):659-68
The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.
Badcock G, Pigott C, Goepel J, Andrews PW
Cancer research 1999 Sep 15;59(18):4715-9
Cancer research 1999 Sep 15;59(18):4715-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Analysis of NB100-730 expression in NTERA-2 cl.D1 whole cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, NBP1-97433) were separated on a 7.5% gel and transferred to PVDF membrane. The membrane was then incubated with anti-TRA1-60(R) antibody at 2 ug/ml and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Note how this antibody specifically detects the TRA1-60(R) epitope only on human cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730] - BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # NB100-730, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).
