AMAb90667

antibody from Atlas Antibodies

Targeting: PODXL Gp200, PC, PCLP

Western blot (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb90667

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb90667, RRID:AB_2665627

Product name

Anti-PODXL

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

LPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKC
EDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISL
ICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEIT
IHTKLPAKDVYERLKDKWDELKEAGVSDMKLGD


Epitope

Binds to an epitope located within the peptide sequence IHTKLPAKDVYERLK as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL0308

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer

Boman K, Larsson A, Segersten U, Kuteeva E, Johannesson H, Nodin B, Eberhard J, Uhlén M, Malmström P, Jirström K
British Journal of Cancer 2013 May;108(11):2321-2328

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Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer.

Boman K, Larsson AH, Segersten U, Kuteeva E, Johannesson H, Nodin B, Eberhard J, Uhlén M, Malmström PU, Jirström K
British journal of cancer 2013 Jun 11;108(11):2321-8

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human kidney and liver tissues using AMAb90667 antibody. Corresponding PODXL RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong positivity in renal glomeruli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong immunoreactivity in the endothelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colorectal tumour shows strong membranous, combined with moderate cytoplasmic, immunoreactivity in tumour cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colorectal tumour shows moderate cytoplasmic immunoreactivity in tumour cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colorectal tumour shows negative tumour cells and positive stromal vessels.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows moderate positivity in apical membranes of glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows weak to moderate membranous positivity in endothelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.