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- Antigen structure
- References [10]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF1658 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Podocalyxin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse Podocalyxin and recombinant human Endoglycan.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAB61574- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Organoid single cell profiling identifies a transcriptional signature of glomerular disease.
Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development.
Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray.
Overexpression of preeclampsia induced microRNA-26a-5p leads to proteinuria in zebrafish.
Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma.
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4.
Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.
Loss of podocalyxin causes a novel syndromic type of congenital nephrotic syndrome.
β-Catenin Is Required for Endothelial Cyp1b1 Regulation Influencing Metabolic Barrier Function.
Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies.
Harder JL, Menon R, Otto EA, Zhou J, Eddy S, Wys NL, O'Connor C, Luo J, Nair V, Cebrian C, Spence JR, Bitzer M, Troyanskaya OG, Hodgin JB, Wiggins RC, Freedman BS, Kretzler M, European Renal cDNA Bank (ERCB), Nephrotic Syndrome Study Network (NEPTUNE)
JCI insight 2019 Jan 10;4(1)
JCI insight 2019 Jan 10;4(1)
Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development.
Hochane M, van den Berg PR, Fan X, Bérenger-Currias N, Adegeest E, Bialecka M, Nieveen M, Menschaart M, Chuva de Sousa Lopes SM, Semrau S
PLoS biology 2019 Feb;17(2):e3000152
PLoS biology 2019 Feb;17(2):e3000152
Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray.
Saito S, Hiemori K, Kiyoi K, Tateno H
Scientific reports 2018 Mar 5;8(1):3997
Scientific reports 2018 Mar 5;8(1):3997
Overexpression of preeclampsia induced microRNA-26a-5p leads to proteinuria in zebrafish.
Müller-Deile J, Schröder P, Beverly-Staggs L, Hiss R, Fiedler J, Nyström J, Thum T, Haller H, Schiffer M
Scientific reports 2018 Feb 26;8(1):3621
Scientific reports 2018 Feb 26;8(1):3621
Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma.
Itai S, Ohishi T, Kaneko MK, Yamada S, Abe S, Nakamura T, Yanaka M, Chang YW, Ohba SI, Nishioka Y, Kawada M, Harada H, Kato Y
Oncotarget 2018 Apr 27;9(32):22480-22497
Oncotarget 2018 Apr 27;9(32):22480-22497
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4.
Volk-Draper LD, Hall KL, Wilber AC, Ran S
PloS one 2017;12(6):e0179257
PloS one 2017;12(6):e0179257
Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.
Morizane R, Bonventre JV
Nature protocols 2017 Jan;12(1):195-207
Nature protocols 2017 Jan;12(1):195-207
Loss of podocalyxin causes a novel syndromic type of congenital nephrotic syndrome.
Kang HG, Lee M, Lee KB, Hughes M, Kwon BS, Lee S, McNagny KM, Ahn YH, Ko JM, Ha IS, Choi M, Cheong HI
Experimental & molecular medicine 2017 Dec 15;49(12):e414
Experimental & molecular medicine 2017 Dec 15;49(12):e414
β-Catenin Is Required for Endothelial Cyp1b1 Regulation Influencing Metabolic Barrier Function.
Ziegler N, Awwad K, Fisslthaler B, Reis M, Devraj K, Corada M, Minardi SP, Dejana E, Plate KH, Fleming I, Liebner S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 24;36(34):8921-35
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 24;36(34):8921-35
Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies.
Ylöstalo J, Bazhanov N, Prockop DJ
Experimental hematology 2008 Oct;36(10):1390-402
Experimental hematology 2008 Oct;36(10):1390-402
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Podocalyxin by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human Podocalyxin Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Podocalyxin/PODXL knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Podocalyxin at approximately 160 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Podocalyxin in Human Kidney. Podocalyxin was detected in immersion fixed paraffin-embedded sections of human kidney using 5 µg/mL Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
