PA5-81077

antibody from Invitrogen Antibodies

Targeting: TNFRSF10D CD264, DcR2, TRAILR4, TRUNDD

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-81077

Provider

Invitrogen Antibodies

Product name

CD264 (TRAIL-R4) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human TRAIL R4/CD264/TNFRSF10D.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD264 (TRAIL-R4) Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 0.5 µL with CD264 (TRAIL-R4) Polyclonal Antibody (Product # PA5-81077) and 15 µL of 50 % Protein G agarose. Primary antibody: CD264 (TRAIL-R4) Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human CD264 (TRAIL-R4) in human liver with CD264 (TRAIL-R4) Polyclonal Antibody (Product # PA5-81077, 1:5,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human CD264 (TRAIL-R4) in human kidney with CD264 (TRAIL-R4) Polyclonal Antibody (Product # PA5-81077, 1:5,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human CD264 (TRAIL-R4) in human testis with CD264 (TRAIL-R4) Polyclonal Antibody (Product # PA5-81077, 1:5,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD264 (TRAIL-R4) Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 0.5 µL with CD264 (TRAIL-R4) Polyclonal Antibody (Product # PA5-81077) and 15 µL of 50 % Protein G agarose. Primary antibody: CD264 (TRAIL-R4) Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.