PA5-34432

antibody from Invitrogen Antibodies

Targeting: CDKN1C BWCR, BWS, KIP2, P57

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34432

Provider

Invitrogen Antibodies

Product name

p57 Kip2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is 293 cell lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of 293 cell lysate using a CDKN1C polyclonal antibody (Product # PA5-34432) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CDKN1C in 293 cell lysate with p57 Kip2 Polyclonal Antibody (Product # PA5-34432) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using p57 Kip2 Polyclonal Antibody (Product # PA5-34432) and a 57 kDa band corresponding to p57KIP2 was observed across cell lines tested except MCF-7 which is reported to be negative. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SK-BR-3 (Lane 2), K-562 (Lane 3), MCF-7 (Lane 4) and OVCAR-3 (Lane 5) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 293 cells using a CDKN1C polyclonal antibody (Product # PA5-34432) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of CDKN1C in 293 cells with p57 Kip2 Polyclonal Antibody (Product # PA5-34432) at 20 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of CDKN1C in 293 cells with p57 Kip2 Polyclonal Antibody (Product # PA5-34432) at 20 µg/mL.