Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [3]
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- Product number
- PA5-34432 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p57 Kip2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is 293 cell lysate. PA5-34432 can be used with blocking peptide PEP-1474.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 293 cell lysate using a CDKN1C polyclonal antibody (Product # PA5-34432) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDKN1C in 293 cell lysate with p57 Kip2 Polyclonal Antibody (Product # PA5-34432) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using p57 Kip2 Polyclonal Antibody (Product # PA5-34432) and a 57 kDa band corresponding to p57KIP2 was observed across cell lines tested except MCF-7 which is reported to be negative. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SK-BR-3 (Lane 2), K-562 (Lane 3), MCF-7 (Lane 4) and OVCAR-3 (Lane 5) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 293 cells using a CDKN1C polyclonal antibody (Product # PA5-34432) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of CDKN1C in 293 cells with p57 Kip2 Polyclonal Antibody (Product # PA5-34432) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of CDKN1C in 293 cells with p57 Kip2 Polyclonal Antibody (Product # PA5-34432) at 20 µg/mL.