OSA00164W-100UL

antibody from Invitrogen Antibodies

Targeting: ATP8B1 ATPIC, BRIC, FIC1, PFIC, PFIC1

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

OSA00164W-100UL

Provider

Invitrogen Antibodies

Product name

ATP8B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IHC on paraffin sections of human muscle tissue (negative control) using Rabbit antibody to ATP8B1:OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1:OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1:OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1:OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1:OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.