NBP2-25092

antibody from Novus Biologicals

Targeting: TNFRSF10A Apo2, CD261, DR4, TRAILR-1, TRAILR1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-25092

Provider

Novus Biologicals

Product name

Rabbit Polyclonal TRAILR1/TNFRSF10A Antibody

Antibody type

Polyclonal

Description

Peptide affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Synergistic antitumor activity of TRAIL combined with chemotherapeutic agents in A549 cell lines in vitro and in vivo.

Fan QL, Zou WY, Song LH, Wei W
Cancer chemotherapy and pharmacology 2005 Feb;55(2):189-96

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Analysis of TRAIL R1/TNFRSF10A in HeLa total cell lysate with TRAIL R1/TNFRSF10A antibody at 0.5 ug/mL.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Analysis of 50 ug of total cell lysate from HeLa cells with anti-DR4 (CT) at 1:500 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Analysis of DR4 in (A) HepG2 and (B) Jurkat cell lysate with DR4 antibody at 1 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 ug of lysates per lane. Antibodies: DR4 NBP2-25092 (1 ug/mL), DR4 NBP1-76473 ( 4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Western Blot Validation in Cell Lines. Loading: 15 ug of cell lysates per lane. Antibodies: DR4 NBP2-25092 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TRAILR1/TNFRSF10A KO cell lysates. Antibodies: TRAILR1/TNFRSF10A [NBP2-25092] (1 ug/ml) and beta-actin [NBP1-76692] (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Immunofluorescence Validation of DR4. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling DR4 with NBP2-25092 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody (red) and DAPI staining (blue). Image showing membrane staining on human spleen cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Staining of human spleen tissue with antibody at 5 ug/mL.