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LearnMA1-41049
antibody from Invitrogen Antibodies
Targeting: TNFRSF10A
Apo2, CD261, DR4, TRAILR-1, TRAILR1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
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- Product number
- MA1-41049 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DR4 Monoclonal Antibody (32A242)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: Hela whole cell extract, HeLa cell lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 32A242
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DR4 in 20 µg total cell lysate from HeLa cells. Samples were incubated in DR4 monoclonal antibody (Product # MA1-41049) using a dilution of 2 µg/mL followed by a goat anti-mouse Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DR4 in 20 µg total cell lysate from HeLa cells. Samples were incubated in DR4 monoclonal antibody (Product # MA1-41049) using a dilution of 2 µg/mL followed by a goat anti-mouse Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-DR4 Monoclonal Antibody (32A242) (Product # MA1-41049) and a 54 kDa band corresponding to Tumor necrosis factor receptor superfamily member 10A was observed across tested samples. Membrane enriched extracts (40 µg lysate) of MDA-MB-231 (Lane 1), T-47D (Lane 2), MCF7 (Lane 3), A549 (Lane 4), HeLa (Lane 5), Caco-2 (Lane 6), IMR-32 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1 µg/mL concentration) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
