MA5-41048

antibody from Invitrogen Antibodies

Targeting: CPM

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-41048

Provider

Invitrogen Antibodies

Product name

Carboxypeptidase M Recombinant Rabbit Monoclonal Antibody (105)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

105

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Carboxypeptidase M Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 µL with Carboxypeptidase M Recombinant Rabbit Monoclonal Antibody (105) (Product # MA5-41048) and 15 µL of 50 % Protein G agarose. Primary antibody: Carboxypeptidase M Recombinant Rabbit Monoclonal Antibody (105), at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Carboxypeptidase M in Lane A: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41048) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 55 kDa, Observed band size: 55 kDa .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Carboxypeptidase M in Lane A: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41048) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 55 kDa, Observed band size: 55 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Carboxypeptidase M Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 µL with Carboxypeptidase M Recombinant Rabbit Monoclonal Antibody (105) (Product # MA5-41048) and 15 µL of 50 % Protein G agarose. Primary antibody: Carboxypeptidase M Recombinant Rabbit Monoclonal Antibody (105), at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa.