Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-28685 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28685, RRID:AB_1913957
- Product name
- Rabbit Polyclonal SET1B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SET1B Antibody [NBP1-28685] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SET1B antibody NBP1-28685 used for IP at 10 ug/mg lysate. SET1B was also immunoprecipitated by rabbit anti-SET1B antibody NBP1-28684, which recognizes an upstream epitope. For blotting immunoprecipitated SET1B, NBP1-28684 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SET1B Antibody [NBP1-28685] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-hSET1B used at a dilution of 1:1,000 (1ug/ml). Detection: DAB