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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00010785-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010785-M01, RRID:AB_464089
- Product name
- WDR4 monoclonal antibody (M01), clone 1F9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant WDR4.
- Antigen sequence
MLLDVAVSPDDRFILTADRDEKIRVSWAAAPHSIE
SFCLGHTEFVSRISVVPTQPGLLLSSSGDGTLRLW
EYRSGRQLHCCHLASLQELVDPQAPQKFAASRIAF
WCQENCVALLCDGTSVVYIFQLDARRQQLVYRQQL
AFQHQVWDVAFEETQGLWVLQDCQEAPLVLYRPVG
DQWQSVPESTVLKKVSGVLRGNWAMLEGSAGADAS
FSSLYKATFDNVTSYLKKKEERLQQQLEKKQRRRS
PPPGPDGHAKKMRPGEATLSC- Isotype
- IgG
- Antibody clone number
- 1F9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- WDR4 monoclonal antibody (M01), clone 1F9 Western Blot analysis of WDR4 expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of WDR4 expression in transfected 293T cell line by WDR4 monoclonal antibody (M01), clone 1F9.Lane 1: WDR4 transfected lysate (Predicted MW: 45.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged WDR4 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to WDR4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
