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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-51645 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMOD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LEKEALEHKD REDYVPYTGE KKGKIFIPKQ KPVQTFTEEK VSLDPELEEA LTSASDTELC DLAAILGMHN LITNTKFCNI MGSSNGVDQE HFSNVVKGEK ILPVFDEPPN PTNVEESLKR TKENDAHLVE VNLNNIKNIP IPTLKD Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 90%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism.
Sommerkamp P, Altamura S, Renders S, Narr A, Ladel L, Zeisberger P, Eiben PL, Fawaz M, Rieger MA, Cabezas-Wallscheid N, Trumpp A
Cell stem cell 2020 May 7;26(5):722-738.e7
Cell stem cell 2020 May 7;26(5):722-738.e7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TMOD3 in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12 using a TMOD3 Polyclonal Antibody (Product # PA5-51645).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TMOD3 in human cell lines A-549 and HEK293 using a TMOD3 Polyclonal Antibody (Product # PA5-51645). Corresponding TMOD3 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TMOD3 in human cell line U-2 OS shows positivity in cytoplasm & actin filaments. Samples were probed using a TMOD3 Polyclonal Antibody (Product # PA5-51645).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TMOD3 in human gallbladder using a TMOD3 Polyclonal Antibody (Product # PA5-51645) shows strong cytoplasmic positivity in glandular cells.
