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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP11506PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11506PU-N, RRID:AB_1748330
- Product name
- anti alpha 1 cardiac muscle Actin / ACTC1
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ACTB/ACTC.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ACTB/ACTC Antibody (Center) in HL60 cell line lysates (35 µg/lane). Target protein (arrow) was detected using the purified Pab (1:60 dilution).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ACTB/ACTC (arrow) using rabbit polyclonal ACTB/ACTC Antibody (Center) (AP11506PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ACTB/ACTC antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
