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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA303378 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA303378, RRID:AB_2257958
- Product name
- Rabbit monoclonal antibody against Actin (Pan)(EP184E)
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody against Actin (Pan)(EP184E)
- Reactivity
- Bovine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ACTC1
- Isotype
- IgG
- Antibody clone number
- EP184E
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - muscle Actin antibody [EP184E]; Anti-muscle Actin antibody [EP184E] at 1/1000 dilution + A431 cell lysate at 10ug/lane.Secondary.Goat anti-rabbit, HRP labelled. at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/ Immunofluorescence - muscle Actin antibody [EP184E]; Immunofluorescent staining of HeLa cells using TA303378 at a 1/250 dilution.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin-embedded sections) - muscle Actin antibody [EP184E]; Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin TA303378 at a 1/100 dilution.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E]; Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using TA303378. Green-specific fluorescent staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen sections) - Anti-muscle Actin antibody [EP184E]; Immunohistochemical analysis of rat kidney tubule tissue, staining muscle Actin with TA303378.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry-Anti-muscle Actin antibody(TA303378); Overlay histogram showing HeLa cells stained with TA303378 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in HeLa cells under the same conditions.
- Validation comment
- FC
