- PA5-21396 - Invitrogen Antibodies ACTC1 antibody

Yin R, Wang H, Li C, Wang L, Lai S, Yang X, Hong D, Zhang W
Cell death discovery 2022 Feb 21;8(1):75

Pourcel L, Buron F, Arib G, Le Fourn V, Regamey A, Bodenmann I, Girod PA, Mermod N
Biotechnology and bioengineering 2020 Apr;117(4):1117-1126

Cervero P, Wiesner C, Bouissou A, Poincloux R, Linder S
Nature communications 2018 Feb 6;9(1):515

Jiang H, Qu P
Experimental and therapeutic medicine 2017 Dec;14(6):5857-5862

Mouton J, Loos B, Moolman-Smook JC, Kinnear CJ
Experimental cell research 2015 Feb 15;331(2):338-51

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody (Product # PA5-21396) diluted at 1:200. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: alpha-Cardiac Actin Polyclonal Antibody detects alpha Cardiac Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. alpha Cardiac Muscle Actin stained by alpha-Cardiac Actin Polyclonal Antibody (Product # PA5-21396) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: alpha-Cardiac Actin Polyclonal Antibody detects alpha Cardiac Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. alpha Cardiac Muscle Actin stained by alpha-Cardiac Actin Polyclonal Antibody (Product # PA5-21396) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 8 Differential subcellular recruitment of LSP1 and supervillin is based on the preferential binding of LSP1 to beta-actin. a Comassie blue staining of gel showing actin cosedimentation assays with pure beta-actin, alpha-cardiac actin, or LSP1 6xHis, alone as control, or in combination with actin isoforms, as indicated. Lanes showing supernatant and pellet fractions are labelled with ""S"" and ""P"", accordingly. Molecular weight is indicated in kDa. b Quantification of copelleted material as ratios of pelleted fraction versus input. Values are given as mean +- S.D.; **** P < 0.0001, two-tailed unpaired t -test. c - h Confocal micrographs of macrophages treated with control siRNA ( c - e ) or beta-actin-specific siRNA ( f - h ), stained for beta-actin ( c , f ), alpha-cardiac actin ( d , g ), and LSP1 ( e , h ). (Note: staining conditions required especially for alpha-cardiac actin are not optimal for staining of LSP1.) Scale bars: 10 um. For specific values, see Supplementary Data 1

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 Characterization of the productivity of ACTC1-overexpressing cells. A trastuzumab-expressing line was stably retransfected with the CHO ACTC1 or with an empty expression vector, and cell clones were isolated for further analysis. (a) Immunoblots of total protein extracts labeled with ACTC1 or GAPDH mouse antibodies. The histogram shows the ratio of the ACTC1 signal relative to that of GAPDH, as assessed using the ImageJ. Ponceau red-stained membranes are shown in Figure S3a. (b) Secreted IgG titers in culture supernatants were assessed by double sandwich ELISA over 13 days of fed-batch cultures. CHO, Chinese hamster ovary; ELISA, enzyme-linked immunosorbent assay; IgG, immunoglobulin gamma [Color figure can be viewed at wileyonlinelibrary.com]

PA5-21396