NB100-1828

antibody from Novus Biologicals

Targeting: NKX3-1 BAPX2, NKX3.1, NKX3A

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1828

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1828, RRID:AB_10001847

Product name

Mouse Monoclonal NKX3.1 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

2.3 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Epigenetic silencing of SALL2 confers tamoxifen resistance in breast cancer.

Ye L, Lin C, Wang X, Li Q, Li Y, Wang M, Zhao Z, Wu X, Shi D, Xiao Y, Ren L, Jian Y, Yang M, Ou R, Deng G, Ouyang Y, Chen X, Li J, Song L
EMBO molecular medicine 2019 Dec;11(12):e10638

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TMPRSS2-ERG Controls Luminal Epithelial Lineage and Antiandrogen Sensitivity in PTEN and TP53-Mutated Prostate Cancer.

Blee AM, He Y, Yang Y, Ye Z, Yan Y, Pan Y, Ma T, Dugdale J, Kuehn E, Kohli M, Jimenez R, Chen Y, Xu W, Wang L, Huang H
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Sep 15;24(18):4551-4565

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p300 acetyltransferase regulates androgen receptor degradation and PTEN-deficient prostate tumorigenesis.

Zhong J, Ding L, Bohrer LR, Pan Y, Liu P, Zhang J, Sebo TJ, Karnes RJ, Tindall DJ, van Deursen J, Huang H
Cancer research 2014 Mar 15;74(6):1870-1880

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Nkx3.1 functions as para-transcription factor to regulate gene expression and cell proliferation in non-cell autonomous manner.

Zhou J, Qin L, Tien JC, Gao L, Chen X, Wang F, Hsieh JT, Xu J
The Journal of biological chemistry 2012 May 18;287(21):17248-56

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A critical role for p27kip1 gene dosage in a mouse model of prostate carcinogenesis.

Gao H, Ouyang X, Banach-Petrosky W, Borowsky AD, Lin Y, Kim M, Lee H, Shih WJ, Cardiff RD, Shen MM, Abate-Shen C
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 7;101(49):17204-9

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Cooperativity of Nkx3.1 and Pten loss of function in a mouse model of prostate carcinogenesis.

Kim MJ, Cardiff RD, Desai N, Banach-Petrosky WA, Parsons R, Shen MM, Abate-Shen C
Proceedings of the National Academy of Sciences of the United States of America 2002 Mar 5;99(5):2884-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Nkx3.1 Antibody (0361) [NB100-1828] - Detection of NKX3.1 in mouse testis lysate using NB 100-1828. ECL detection 1 minute.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NKX3.1 Antibody (0361) [NB100-1828] - analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. Image from verified customer review.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - NKX3.1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Mouse Anti-Mouse NKX3.1 (0361) Monoclonal Antibody (Catalog # NB100-1828) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei in sperm cells.