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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP51433PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51433PU-N, RRID:AB_11146346
- Product name
- anti CD249 / Glutamyl aminopeptidase (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 906-938 amino acids from the C-terminal region of Human ENPEP.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of CD249 / Glutamyl aminopeptidase Antibody (C-term) Cat.-No AP51433PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the ENPEP antibody detected the ENPEP protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human kidney tissue reacted with CD249 / Glutamyl aminopeptidase Antibody (C-term) Cat.-No AP51433PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using CD249 / Glutamyl aminopeptidase Antibody (C-term) Cat.-No AP51433PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
