MA1-06821

antibody from Invitrogen Antibodies

Targeting: HSPG2 perlecan, PRCAN, SJS1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-06821

Provider

Invitrogen Antibodies

Product name

Perlecan Monoclonal Antibody (A7L6)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-06821 detects large heparan sulphate proteoglycan in human, mouse, bovine, cod, wolffish, and rat samples. MA1-06821 has sucessfully been used in Western blotting, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot it detects a >400 kDa band representing large HSPG. The MA1-06821 immunogen is a high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

Reactivity

Human, Mouse, Rat, Bovine

Host

Rat

Isotype

IgG

Antibody clone number

A7L6

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition.

Guo D, Ru J, Mao F, Ouyang H, Ju R, Wu K, Liu Y, Liu C
Development (Cambridge, England) 2020 Nov 30;147(22)

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Adipocyte-Derived Versican and Macrophage-Derived Biglycan Control Adipose Tissue Inflammation in Obesity.

Han CY, Kang I, Harten IA, Gebe JA, Chan CK, Omer M, Alonge KM, den Hartigh LJ, Gomes Kjerulf D, Goodspeed L, Subramanian S, Wang S, Kim F, Birk DE, Wight TN, Chait A
Cell reports 2020 Jun 30;31(13):107818

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Reactivating the extracellular matrix synthesis of sulfated glycosaminoglycans and proteoglycans to improve the human skin aspect and its mechanical properties.

Chajra H, Auriol D, Joly F, Pagnon A, Rodrigues M, Allart S, Redziniak G, Lefevre F
Clinical, cosmetic and investigational dermatology 2016;9:461-472

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PDGFRβ regulates craniofacial development through homodimers and functional heterodimers with PDGFRα.

Fantauzzo KA, Soriano P
Genes & development 2016 Nov 1;30(21):2443-2458

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Liver aging and pseudocapillarization in a Werner syndrome mouse model.

Cogger VC, Svistounov D, Warren A, Zykova S, Melvin RG, Solon-Biet SM, O'Reilly JN, McMahon AC, Ballard JW, De Cabo R, Le Couteur DG, Lebel M
The journals of gerontology. Series A, Biological sciences and medical sciences 2014 Sep;69(9):1076-86

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LARGE glycans on dystroglycan function as a tunable matrix scaffold to prevent dystrophy.

Goddeeris MM, Wu B, Venzke D, Yoshida-Moriguchi T, Saito F, Matsumura K, Moore SA, Campbell KP
Nature 2013 Nov 7;503(7474):136-40

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DOT1L regulates dystrophin expression and is critical for cardiac function.

Nguyen AT, Xiao B, Neppl RL, Kallin EM, Li J, Chen T, Wang DZ, Xiao X, Zhang Y
Genes & development 2011 Feb 1;25(3):263-74

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The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.

Nigro J, White JF, Ramshaw JA, Haylock DN, Nilsson SK, Werkmeister JA
Biomaterials 2010 Jul;31(21):5689-99

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Synthesis of syndecan-1 by skeletal muscle cells is an early response to infection with Trichinella spiralis but is not essential for nurse cell development.

Beiting DP, Park PW, Appleton JA
Infection and immunity 2006 Mar;74(3):1941-3

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunostaining of large heparin sulphate proteoglycan in human skin using Product # MA1-06821.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HSPG2 was performed using 70% confluent log phase HUVEC and SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Perlecan Monoclonal Antibody (A7L6) (Product # MA1-06821, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A48269, 1:2,000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing golgi-like localization. Panel e represents SH-SY5Y cells with lower HSPG2 expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry on frozen section of human kidney showing strong reactivity in the extracellular matrix and basement membrane. Stained with Large Heparan Sulphate Proteoglycan monoclonal antibody (Product # MA1-06821).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Perlecan in frozen sections of human colon using a Perlecan Monoclonal Antibody (A7L6) (Product # MA1-06821), showing strong reactivity in the extracellular matrix and basement membrane.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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