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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- CF504135 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CST4 Monoclonal Antibody (OTI2H10), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2H10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from Hela cell line by using anti-CST4 monoclonal antibody at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CST4 (RC209349, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CST4. Positive lysates LY400707 (100 µg) and LC400707 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 1 cell line by using anti-CST4 monoclonal antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-CST4 mouse monoclonal antibody (TA504135).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209349 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CST4 antibody (TA504135), and then analyzed by flow cytometry.
