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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [4]
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- Product number
- PA5-51722 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF1AX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KRELVFKEDG QEYAQVIKML GNGRLEALCF DGVKRLCHIR GKLRKKVWIN TSDIILVGLR DYQDNKADVI LKYNADEARS LKAYGELPEH AKINETDTFG PGDDDEIQ Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF1AX in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an EIF1AX Polyclonal Antibody (Product # PA5-51722).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF1AX in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Mouse cerebral cortex tissue. Samples were probed using an EIF1AX Polyclonal Antibody (Product # PA5-51722).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF1AX in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells); Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla). Samples were probed using an EIF1AX Polyclonal Antibody (Product # PA5-51722).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of eIF1AX in human skeletal muscle using eIF1AX Polyclonal Antibody (Product # PA5-51722) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of eIF1AX in human endometrium using eIF1AX Polyclonal Antibody (Product # PA5-51722) shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of eIF1AX in human testis using eIF1AX Polyclonal Antibody (Product # PA5-51722) shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of eIF1AX in human cerebral cortex using eIF1AX Polyclonal Antibody (Product # PA5-51722) shows moderate cytoplasmic positivity in neurons.
