H00008490-M01

antibody from Abnova Corporation

Targeting: RGS5

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008490-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008490-M01, RRID:AB_1112251

Product name

RGS5 monoclonal antibody (M01), clone 4E12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RGS5.

Antigen sequence

WIACEDYKKIKSPAKMAEKAKQIYEEFIQTEAPKE
VNIDHFTKDITMKNLVEPSLSSFDMAQKRIHALME
KDSLPRFVRSEFYQELIK


Isotype

IgG

Antibody clone number

4E12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 monoclonal antibody (M01), clone 4E12.Lane 1: RGS5 transfected lysate (Predicted MW: 20.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RGS5 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol