MAB6838

antibody from Novus Biologicals

Targeting: SFRP2 FRP-2, SARP1, SDF-5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6838

Provider

Novus Biologicals

Product name

Mouse Monoclonal sFRP-2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human sFRP-2 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) sFRP-2 is observed and no cross-reactivity with recombinant human (rh) sFRP-1 or rhsFRP-5 is observed. In Western blots, no cross-reactivity with rhsFRP-1, -4, -5, or rmsFRP-2 is observed. 100% cross-reactivity with rhsFRP-3 is observed under non-reducing conditions only.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance.

Kaur A, Webster MR, Marchbank K, Behera R, Ndoye A, Kugel CH 3rd, Dang VM, Appleton J, O'Connell MP, Cheng P, Valiga AA, Morissette R, McDonnell NB, Ferrucci L, Kossenkov AV, Meeth K, Tang HY, Yin X, Wood WH 3rd, Lehrmann E, Becker KG, Flaherty KT, Frederick DT, Wargo JA, Cooper ZA, Tetzlaff MT, Hudgens C, Aird KM, Zhang R, Xu X, Liu Q, Bartlett E, Karakousis G, Eroglu Z, Lo RS, Chan M, Menzies AM, Long GV, Johnson DB, Sosman J, Schilling B, Schadendorf D, Speicher DW, Bosenberg M, Ribas A, Weeraratna AT
Nature 2016 Apr 14;532(7598):250-4

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human sFRP-2 by Western Blot. Western blot shows lysates of human adipose tissue and human lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human sFRP-2 Monoclonal Antibody (Catalog # MAB6838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for sFRP-2 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.