Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX111892 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111892, RRID:AB_2037978
- Product name
- SFRP2 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) B: HeLa 12% SDS PAGE GTX111892 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using FRP-2 (GTX111892) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SFRP2 antibody [C2C3], C-term detects SFRP2 protein by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SFRP2 protein stained by SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using FRP 2(GTX111892) antibody at 1:500 dilution.