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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29390 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SFRP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, HeLa. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Dog (100%), Pig (100%), Sheep (100%), Chimpanzee (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.12 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Oncogenic role of SFRP2 in p53-mutant osteosarcoma development via autocrine and paracrine mechanism.
Kim H, Yoo S, Zhou R, Xu A, Bernitz JM, Yuan Y, Gomes AM, Daniel MG, Su J, Demicco EG, Zhu J, Moore KA, Lee DF, Lemischka IR, Schaniel C
Proceedings of the National Academy of Sciences of the United States of America 2018 Nov 20;115(47):E11128-E11137
Proceedings of the National Academy of Sciences of the United States of America 2018 Nov 20;115(47):E11128-E11137
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FRP 2 using 30 µg of A) A431 and B) HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a FRP 2 polyclonal antibody (Product # PA5-29390) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SFRP2 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SFRP2 Polyclonal Antibody (Product # PA5-29390) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SFRP2 Polyclonal Antibody (Product # PA5-29390). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SFRP2 Polyclonal Antibody (Product # PA5-29390) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SFRP2 was performed in HCT 116 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SFRP2 Polyclonal Antibody (Product # PA5-29390) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using FRP 2 (Product # PA5-29390) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
