Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-35973 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Secretin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: unboiled DDDDK-tagged SCT-transfected 293T cells. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Secretin using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of DDDDK-human SCT-transfected 293T cells. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Secretin polyclonal antibody (Product # PA5-35973) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Secretin was performed by separating 30 µg of non-transfected (–) and transfected (+) unboiled 293T whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Secretin Polyclonal Antibody (Product # PA5-35973) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.